Home-cage monitoring spontaneous activity of C57BL/6J male mice 3 months after open-field low-intensity blast exposure

DOI:10.34945/F5FK5C

DATASET CITATION

Zuckerman A., Siedhoff H. R., Balderrama A., Cui J., Gu Z. (2023) Home-cage monitoring spontaneous activity of C57BL/6J male mice 3 months after open-field low-intensity blast exposure. Open Data Commons for Traumatic Brain Injury. ODC-TBI:871 http://doi.org/10.34945/F5FK5C

ABSTRACT

STUDY PURPOSE: Evaluate the chronic-phase behavioral alterations 3 months after exposure to low-intensity blast in a home-cage-like environment.

DATA COLLECTED: A total of 52 male C57Bl/6J mice, 8 weeks old, were used. The mice were randomly allocated into one of two groups: Blast (n=29) or Sham (n=23). Mice in the Blast group were exposed to open-field low-pressure blast wave (46.6 kPa, maximum impulse of 60.0 kPa*ms), under anesthesia. Mice from the Sham group were anesthetized but were not exposed to the blast wave. 3 months post-exposure, the spontaneous activity of the mice was measured using the PhenoTyper® home-cages (L = 30 × W = 30 × H = 35 cm; Model 3000, Noldus Information Technology, The Netherlands). Each mouse was housed individually, and its activity was continuously measured for 72 hours at a sample rate of 15 fps. Program-acquired data were uploaded to the web-based AHCODA-DB (Sylics, Bilthoven, The Netherlands) for meta-analysis. Twenty behavioral parameters were analyzed and included in this dataset. See protocols and other related data in the relevant links section below.

CONCLUSIONS: No significant differences were found between the Blast and Sham mice in different parameters of general daily performance behaviors, such as activity, arrests, and feeding zone visits. Although no significant difference in long shelter visits between the Blast and Sham mice, was found, significant differences were found in multiple parameters of short shelter visits such as “shelter visit threshold” and “short shelter visit duration” (relevant to anxiety-like behaviors). Blast mice visited their shelters more frequently and for shorter periods of time than Sham mice in both dark and light phases. These results suggest that LIB-exposed mice may hold stable perceptions of environmental stimuli as a threat during activity bouts, whereas sham controls experienced such responses to a lesser degree. This type of performance is consistent as trait anxiety in humans, defined as a tendency to respond with concerns, troubles, and worries to non-threatening situations.

KEYWORDS

primary open-field blast; home-cage monitoring; PhenoTyper; spontaneous activity; anxiety-like behaviors

PROVENANCE / ORIGINATING PUBLICATIONS

• Siedhoff HR, Chen S, Balderrama A, Sun GY, Koopmans B, DePalma RG, Cui J, Gu Z. Long-Term Effects of Low-Intensity Blast Non-Inertial Brain Injury on Anxiety-Like Behaviors in Mice: Home-Cage Monitoring Assessments. Neurotrauma Reports. Jan 2022. 3.1:27-38.. doi:10.1089/neur.2021.0063.

RELEVANT LINKS

Home-cage monitoring general behavior of C57BL/6J male mice during the CognitionWall test 3 months after open-field LIB exposure

https://dx.doi.org/10.34945/F59W23

Releted dataset in ODC-TBI

Open-field blast (OFB) model in mice protocol

https://dx.doi.org/10.17504/protocols.io.yxmvm2kwog3p/v1

Protocol for the Open-field blast (OFB) model in mice in protocols.io

Open-field Blast parameters dataset

https://dx.doi.org/10.34945/F5630G

Datasets with the blast parameters for the relevant subjects

NOTES

DATASET INFO

Contact: Gu Zezong (guze@health.missouri.edu)

Lab: PRECISE-TBI Lab: Truman Memorial VA

ODC-TBI Accession:871

Records in Dataset: 3744

Fields per Record: 26

Last updated: 2023-06-09

Date published: 2023-06-09

Downloads: 10

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

Department of Veterans Affairs Offices of Research & Development (VA ORD) LAMb/ShEEP programs, BLR&D Director Service program UFR-002-18F, Open-Field Blast (OFB) Core, and the Collaborative Merit Review for TBI Research Program I01 BX004313-01A1 (ZG), DoD Congressionally Directed Medical Research Programs (CDMRP) for the Peer Reviewed Alzheimer’s Research Program Convergence Science Research Award PRARPCSRA; AZ180043 (ZG), Research funds of the University of Missouri to ZG.

CONTRIBUTORS

Zuckerman, Amitai [ORCID:0000-0002-7075-1913]

Harry S. Truman Memorial Veterans’ Hospital Research Service, Columbia, Missouri, USA; Department of Pathology and Anatomical Sciences, University of Missouri School of Medicine, Columbia, Missouri, USA

Siedhoff, Heather R.

Harry S. Truman Memorial Veterans’ Hospital Research Service, Columbia, Missouri, USA; Department of Pathology and Anatomical Sciences, University of Missouri School of Medicine, Columbia, Missouri, USA

Balderrama, Ashley

Harry S. Truman Memorial Veterans’ Hospital Research Service, Columbia, Missouri, USA; Department of Pathology and Anatomical Sciences, University of Missouri School of Medicine, Columbia, Missouri, USA

Cui, Jiankun

Harry S. Truman Memorial Veterans’ Hospital Research Service, Columbia, Missouri, USA; Department of Pathology and Anatomical Sciences, University of Missouri School of Medicine, Columbia, Missouri, USA

Gu, Zezong [ORCID:0000-0002-2411-7460]

Harry S. Truman Memorial Veterans’ Hospital Research Service, Columbia, Missouri, USA; Department of Pathology and Anatomical Sciences, University of Missouri School of Medicine, Columbia, Missouri, USA