Traumatic Brain Injury Induces Region-specific Glutamate Metabolism Changes As Measured by Multiple Mass Spectrometry Methods

DOI:10.34945/F5W011

DATASET CITATION

Sowers J. L., Sowers M. L., Zhang K., Hawkins B. E. (2021) Traumatic Brain Injury Induces Region-specific Glutamate Metabolism Changes As Measured by Multiple Mass Spectrometry Methods. ODC-TBI:671 http://doi.org/10.34945/F5W011

ABSTRACT

STUDY PURPOSE: The purpose of this study was to investigate the early metabolic events occurring in the acute phase of traumatic brain injury (TBI) using multiple mass spectrometry methods

DATA COLLECTED: Data collected was tandem mass tag (TMT) multiplexed based proteomic data acquired from the cortex and hippocampus of injured, sham, and naïve rats. Injured rats were subjected to surgery followed by a fluid percussion injury while sham animals only had surgery. Data was collected at five time points after TBI: 24 hours, 2 weeks, 3 months, 6 months and 1 year. For this study, “Traumatic Brain Injury Induces Region-specific Glutamate Metabolism Changes As Measured by Multiple Mass Spectrometry Methods,” only select proteins from the 24 hour and 2 week time points were used

CONCLUSIONS: We revealed new insights and potential pathways for intervention in acute TBI

KEYWORDS

Traumatic brain injury; glutamate oxidation; glutamate metabolism; glutamate excitotoxicity; MALDI-MS imaging; energy failure; nitric oxide

PROVENANCE / ORIGINATING PUBLICATIONS

• James L. Sowers, Mark L. Sowers, Alexander S. Shavkunov, Bridget E. Hawkins, Ping Wu, Douglas S. DeWitt, Donald S. Prough, Kangling Zhang, Traumatic brain injury induces region-specific glutamate metabolism changes as measured by multiple mass spectrometry methods, iScience, Volume 24, Issue 10, 2021, 103108, ISSN 2589-0042. doi:10.1016/j.isci.2021.103108.

Originating publication

RELEVANT LINKS

Traumatic Brain Injury Induces Region-specific Glutamate Metabolism Changes As Measured by Multiple Mass Spectrometry Methods (Animal Metadata)

https://odc-tbi.org/data/670

Associated metadata per animal

NOTES

DATASET INFO

Contact: Hawkins Bridget (behawkin@utmb.edu)

Lab: Moody Project Consortium

ODC-TBI Accession:671

Records in Dataset: 781997

Fields per Record: 491

Last updated: 2021-10-07

Date published: 2021-10-07

Downloads: 30

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

The Moody Project for Translational Traumatic Brain Injury Research (all Authors; PI DeWitt and Prough), UTMB Department of Pharmacology and Toxicology (JLS, MLS and KZ), UTMB Department of Anesthesiology (BEH), UTMB MD-PhD combined degree program (JLS and MLS)

CONTRIBUTORS

Sowers, James L. [ORCID:0000-0002-1442-9882]

MD-PhD Combined Degree Program, University of Texas Medical Branch, Galveston, Texas, USA. Department of Neuroscience, Cell Biology, and Anatomy, University of Texas Medical Branch, Galveston, Texas, USA. The Moody Project for Translational Traumatic Brain Injury Research, University of Texas Medical Branch, Galveston, Texas, USA.

Sowers, Mark L. [ORCID:0000-0001-6474-017X]

MD-PhD Combined Degree Program, University of Texas Medical Branch, Galveston, Texas, USA. Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA.

Zhang, Kangling [ORCID:0000-0003-2151-9218]

Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA. The Moody Project for Translational Traumatic Brain Injury Research, University of Texas Medical Branch, Galveston, Texas, USA.

Hawkins, Bridget E. [ORCID:0000-0002-1887-3657]

Department of Anesthesiology, University of Texas Medical Branch, Galveston, Texas, USA. School of Nursing, University of Texas Medical Branch, Galveston, Texas, USA. The Moody Project for Translational Traumatic Brain Injury Research, University of Texas Medical Branch, Galveston, Texas, USA.