Peripheral monocyte and resident microglia number and cytokine expression at subchronic timepoints after controlled cortical impact TBI in adult and aged mice

DOI:10.34945/F5T595

DATASET CITATION

Chou A., Krukowski K., Morganti J. M., Riparip L. K., Rosi S. (2022) Peripheral monocyte and resident microglia number and cytokine expression at subchronic timepoints after controlled cortical impact TBI in adult and aged mice.. ODC-TBI:376 http://doi.org/10.34945/F5T595

ABSTRACT

STUDY PURPOSE: The purpose of the study was to investigate the effect of age on the inflammatory response of peripheral, infiltrating monocytes after traumatic brain injury.

DATA COLLECTED: The dataset includes several experiments with mice using the parietal controlled cortical impact (CCI) TBI model. A summary of experiments are as follows: (1) Amount of infiltrating monocytes (CD11b+, CD45hi) in the injured brain of wildtype adult (3-4 mo) and aged (18+ mo) mice at 4 and 7 days post-injury (dpi) measured by flow cytommetry. (2) Amount of infiltrating monocyte (CD11b+, CCR2-rfp+) in the injured brain and in the blood of adult and aged (22-24 mo) CCR2-rfp-labeled mice at 4 and 7 dpi measured by flow cytommetry. (3) Amount of newly proliferated infiltrating monocytes (CD11b+, F4/80+, CD45hi) and resident microglia (CD11b+, F4/80+, CD45lo) in the injured brain labeled with BrdU between 3-4 dpi. (4) CCL2, CCL7, CCL8, and CCL12 quantified by qPCR from adult and aged animals at 4 and 7 dpi (normalized to young sham controls). (5) IL-1B, TNFa, iNOS, Ym1, CD206, TGF-B, and IL4Ra inflammatory marker expression measured by qPCR in wildtype adult and aged, Sham and TBI mice at 7 dpi. (6) Radial arm water maze behavior (errors; RAWM) measured at 26-28 dpi in wildtype and CCR2 knockout adult and aged (16-18 mo), Sham and TBI mice. (7) Amount of newly proliferated infiltrating monocytes (CCR2-rfp+) in the blood and bone marrow of adult and aged CCR2-rfp-labeled mice at 4 dpi using EdU labeling and flow cytometry.

CONCLUSIONS:

KEYWORDS

Traumatic brain injury; Aging; Monocyte; Peripheral monocyte; Inflammation; Cognition

PROVENANCE / ORIGINATING PUBLICATIONS

• Chou A, Krukowski K, Morganti JM, Riparip LK, Rosi S. Persistent Infiltration and Impaired Response of Peripherally-Derived Monocytes after Traumatic Brain Injury in the Aged Brain. Int J Mol Sci. 2018;19(6):1616. Published 2018 May 30. doi:10.3390/ijms19061616

Originating publication.

RELEVANT LINKS

NOTES

DATASET INFO

Contact: Chou Austin (austin.chou@ucsf.edu), Rosi Susanna (susanna.rosi@ucsf.edu)

Lab: TOP-NT UCSF

ODC-TBI Accession:376

Records in Dataset: 274

Fields per Record: 42

Last updated: 2022-04-20

Date published: 2022-04-20

Downloads: 56

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

NIA grants R21AG042016 (SR) and F32AG054126 (KK), NIH/NINDS R21NS087458 (SR), NIH/NIA R01AG056770 (SR)

CONTRIBUTORS

Chou, Austin [ORCID:0000-0003-4328-5811]

Brain and Spinal Injury Center, University of California (UCSF)

Krukowski, Karen [ORCID:0000-0003-0281-8917]

Brain and Spinal Injury Center, University of California (UCSF)

Morganti, Josh M. [ORCID:0000-0002-4183-0049]

Brain and Spinal Injury Center, University of California (UCSF)

Riparip, Lara-Kirstie K.

Brain and Spinal Injury Center, University of California (UCSF)

Rosi, Susanna [ORCID:0000-0002-9269-3638]

Brain and Spinal Injury Center, University of California (UCSF)