Humoral and neuronal impacts of traumatic brain injury on fracture healing in mice (polytrauma study)
DOI:10.34945/F58596
DATASET CITATION
Morioka K., Marmor Y., Sacramento J. A., Lin A., Shao T., Miclau K. R., Clark D. R., Beattie M. S., Marcucio R. S., Miclau 3rd T., Ferguson A. R., Bresnahan J. C., Bahney C. S. (2021) Humoral and neuronal impacts of traumatic brain injury on fracture healing in mice (polytrauma study). ODC-TBI:340 http://doi.org/10.34945/F58596
ABSTRACT
STUDY PURPOSE: The aim of this polytraumatic study is to evaluate mechanisms that may influence healing in a preclinical murine model of combined fracture and traumatic brain injury (TBI) compared to fracture or TBI alone.
DATA COLLECTED: Experimental Design:
-4 experimental injury conditions (Tibia Fx Only, CCI TBI Only, Ipsilateral Polytrauma, and Contralateral Polytrauma)
-Controlled Cortical Impact parameters: 3.0 mm rounded head, 21 degrees, 2.0mm deep, 150ms dwell time, and 4 m/s velocity
-3 terminal time points (5, 10, and 14 days post-injury)
Outcomes:
1. Quantification of fracture healing: The volume and the composition of the fracture callus, bone, cartilage, vascular tissue, and fibrous tissue were quantified using a microscope and stereology system.
2. Complete blood count: White blood cells, red blood cells, and thrombocytes were counted in cardiac blood.
3. Systemic inflammation: Inflammatory markers (TNF-alpha, IL-1 beta, IL-10, and Arginine) were evaluated by quantitative RT-PCR in spleen tissues.
4. Quantification of lesion area and volume of brain lesion: Gross brain lesion area and brain lesion volume were assessed using a microscope and imaging software.
5. Multivariate analysis of principal component solution: All outcome measures among three fracture groups (Fx Only, Ipsilateral Polytrauma, and Contralateral Polytrauma) at 2-time points ((5 and 10 days post-injury) were integrated into a principal component analysis (PCA). The three first principal components (PC1, PC2, and PC3) were obtained for the interpretation of PC content based on significant PC loading values.
CONCLUSIONS: Our results suggest that a contralateral bone fracture and TBI alter the neuroinflammatory state to accelerate early fracture healing.
KEYWORDS
Polytrauma; Traumatic brain injury; Bone fracture; PCA
PROVENANCE / ORIGINATING PUBLICATIONS
Morioka K, Marmor Y, Sacramento JA, Lin A, Shao T, Miclau KR, Clark DR, Beattie MS, Marcucio RS, Miclau T 3rd, Ferguson AR, Bresnahan JC, Bahney CS. Differential fracture response to traumatic brain injury suggests dominance of neuroinflammatory response in polytrauma. Sci Rep. 2019 Aug 21;9(1):12199. doi: 10.1038/s41598-019-48126-z. PMID: 31434912; PMCID: PMC6704103.
Originating publication
NOTES
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DATASET INFO
Contact: Bahney Chelsea (cbahney@sprivail.org)
Lab: Ferguson TBI Lab
ODC-TBI Accession:340
Records in Dataset: 64
Fields per Record: 33
Last updated: 2021-08-03
Date published: 2021-08-03
Downloads: 25
Files: 2
LICENSE
Creative Commons Attribution License (CC-BY 4.0)
FUNDING AND ACKNOWLEDGEMENTS
NIAMS NIH grants R21-AR066847 (TM & JCB) and P30 AR066262 (JCL), NINDS NIH grants UG3 NS106899 (ARF) and UG3/UH3NS106899 (ARF), RRD VA I01 RX002787 (ARF), NCI NIH P30 CA082103, NIH NINDS grants U24NS122732 (ARF) and R01NS122888 (ARF)
CONTRIBUTORS
- Morioka, Kazuhito [ORCID:0000-0001-5407-8178]
- Department of Neurological Surgery & Orthopaedic Surgery, University of California, San Francisco (UCSF)
- Marmor, Yotvat
- Department of Orthopaedic Surgery, University of California, San Francisco (UCSF)
- Sacramento, Jeffrey A.
- Department of Neurological Surgery, University of California, San Francisco (UCSF)
- Lin, Amity
- Department of Neurological Surgery, University of California, San Francisco (UCSF)
- Shao, Tiffany
- Department of Orthopaedic Surgery, University of California, San Francisco (UCSF)
- Miclau, Katherine R.
- Department of Orthopaedic Surgery, University of California, San Francisco (UCSF)
- Clark, Daniel R.
- Department of Orthopaedic Surgery, University of California, San Francisco (UCSF)
- Beattie, Michael S.
- Department of Neurological Surgery, University of California, San Francisco (UCSF)
- Marcucio, Ralph S.
- Department of Orthopaedic Surgery, University of California, San Francisco (UCSF)
- Miclau 3rd, Theodore
- Department of Orthopaedic Surgery, University of California, San Francisco (UCSF)
- Ferguson, Adam R.
- Department of Neurological Surgery, University of California, San Francisco (UCSF)
- Bresnahan, Jacqueline C.
- Department of Neurological Surgery, University of California, San Francisco (UCSF)
- Bahney, Chelsea S.
- Department of Orthopaedic Surgery, University of California, San Francisco (UCSF)
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