Cytokine expression and peripheral monocyte infiltration time course and effect of CCR2-antagonist treatment on chronic behavioral outcome in wildtype adult mice after controlled cortical impact TBI
DOI:10.34945/F5PC77
DATASET CITATION
Chou A., Morganti J. M., Jopson T. D., Liu S., Riparip L. K., Guandique C. K., Gupta N., Ferguson A. R., Rosi S. (2022) Cytokine expression and peripheral monocyte infiltration time course and effect of CCR2-antagonist treatment on chronic behavioral outcome in wildtype adult mice after controlled cortical impact TBI.. ODC-TBI:159 http://doi.org/10.34945/F5PC77
ABSTRACT
STUDY PURPOSE: The study was designed to study the role of peripheral infiltrating monocytes to traumatic brain injury induced neuroinflammation and outcome.
DATA COLLECTED: The dataset includes several cohorts of wildtype or CX3CR1(gfp/+)/CCR2(rfp/+) transgenic adult (6-7 mo) mice. TBI was induced using the controlled cortical impact model. (1) Transgenic mice were given sham or TBI surgery and samples were collected at 3 hours, 6 hours, 12 hours, 24 hours, 48 hours, 7 days, 14 days, or 28 days post-injury (dpi). Infiltrating monocytes (CD11b+,F4/80+, CCR2-rfp+, CX3CR1-gfp- and CD11b+,F4/80+, CCR2-rfp+, CX3CR1-gfp+) and resident microglia (CD11b+,F4/80+, CCR2-rfp-, CX3CR1-gfp+) numbers were measured by flow cytometry. 14 inflammatory markers were measured from isolated leukocytes in the injured brain via qPCR. (2) Wildtype adult mice were treated with vehicle or a CCR2-antagonist (CCX872). Infiltrating monocytes (CD11b+, CD45hi) were measured by flow cytometry and 18 inflammatory and oxidative stress molecule expression was measured by qPCR at 1 day post-injury (1 dpi). (3) Wildtype mice treated with vehicle or CCR2-antagonist were measured on radial arm water maze (RAWM) performance at 28 dpi.
CONCLUSIONS:
KEYWORDS
Infiltrating monocyte; Macrophage; Inflammation; Principal Components Analysis; Traumatic brain injury; Cytokine
PROVENANCE / ORIGINATING PUBLICATIONS
Morganti JM, Jopson TD, Liu S, Riparip LK, Guandique CK, Gupta N, Ferguson AR, Rosi S. CCR2 antagonism alters brain macrophage polarization and ameliorates cognitive dysfunction induced by traumatic brain injury. J Neurosci. 2015 Jan 14;35(2):748-60. doi: 10.1523/JNEUROSCI.2405-14.2015. PMID: 25589768; PMCID: PMC4293420.
Originating publication.
RELEVANT LINKS
NOTES
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DATASET INFO
Contact: Rosi Susanna (susanna.rosi@ucsf.edu)
Lab: TOP-NT UCSF
ODC-TBI Accession:159
Records in Dataset:
Fields per Record:
Last updated: 2022-04-20
Date published: 2022-04-20
Downloads: 28
Files: 2
LICENSE
Creative Commons Attribution License (CC-BY 4.0)
FUNDING AND ACKNOWLEDGEMENTS
NINDS F32NS090805 (JMM), NIH/NINDS R21NS087458 (SR), NIH/NIA R21AG042016 (SR), NIH/NCI R01CA133216 (SR), Alzheimer's Association IIRG-11-202064 (SR)
CONTRIBUTORS
- Chou, Austin [ORCID:0000-0003-4328-5811]
- Brain and Spinal Injury Center, University of California, San Francisco (UCSF)
- Morganti, Josh M. [ORCID:0000-0002-4183-0049]
- Brain and Spinal Injury Center, Departments of Physical Therapy and Rehabilitation Science, University of California, San Francisco (UCSF)
- Jopson, Timothy D.
- Brain and Spinal Injury Center, University of California, San Francisco (UCSF)
- Liu, Sharon
- Neurological Surgery, University of California, San Francisco (UCSF)
- Riparip, Lara-Kirstie K.
- Brain and Spinal Injury Center, University of California, San Francisco (UCSF)
- Guandique, Cristian K.
- Brain and Spinal Injury Center, University of California, San Francisco (UCSF)
- Gupta, Nalin [ORCID:0000-0001-9539-7052]
- Neurological Surgery, University of California, San Francisco (UCSF)
- Ferguson, Adam R R. [ORCID:0000-0001-7102-1608]
- Brain and Spinal Injury Center, Neurological Surgery, University of California, San Francisco (UCSF)
- Rosi, Susanna [ORCID:0000-0002-9269-3638]
- Brain and Spinal Injury Center, Departments of Physical Therapy and Rehabilitation Science, and Neurological Surgery, University of California, San Francisco (UCSF)
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