Quantifying acute changes in neurometabolism following blast-induced traumatic brain injury in male Sprague Dawley rats

DOI:10.34945/F5BP43

DATASET CITATION

Norris C., Murphy S. F., VandeVord P. J., Weatherbee J. (2024) Quantifying acute changes in neurometabolism following blast-induced traumatic brain injury in male Sprague Dawley rats. Open Data Commons for Traumatic Brain Injury. ODC-TBI:1014 http://doi.org/10.34945/F5BP43

ABSTRACT

STUDY PURPOSE: The purpose of this study was to optimize and validate methods measuring brain metabolite concentrations in naïve male Sprague Dawley rats and then utilize these methods to detect changes in regional metabolite concentrations at 4 hours following blast exposure.

DATA COLLECTED: Data was collected using high performance liquid chromatography (HPLC) with electrochemical detection. Methods were optimized to quantify alanine, arginine, aspartate, serine, taurine, threonine, tyrosine, glycine, glutamate, glutamine, and gamma-Aminobutyric acid (GABA) in the serum, cerebrospinal fluid, hippocampus, cortex, and cerebellum of 10 week old naïve male Sprague Dawley rats (n = 6). A subsequent preliminary study was performed investigating acute changes in free amino acid concentrations following blast-induced traumatic brain injury (bTBI). Rats were exposed to a single head-on blast wave averaging 126 kPa using an Advanced Blast Simulator. HPLC analytic methods were employed to statistically compare changes in sham (n = 4) and blast (n = 4) concentrations of these metabolites at 4 hours post-injury in the hippocampus and cortex regions.

CONCLUSIONS: Molecular precursors (alanine, arginine, serine, glutamine) decreased in concentration on average by about 10-15% at 4 hours following blast exposure in both the cortex and hippocampus while neurotransmitter concentrations (glycine, taurine, aspartate, glutamate, GABA) remained unchanged relative to the sham group.

KEYWORDS

Blast; Neurotransmitters; Molecular precursors; Neurometabolic; HPLC

PROVENANCE / ORIGINATING PUBLICATIONS

• Norris C, Weatherbee J, Murphy SF, VandeVord PJ. Quantifying acute changes in neurometabolism following blast-induced traumatic brain injury. Neurosci Res. 2023 Jun 21:S0168-0102(23)00130-X. doi: 10.1016/j.neures.2023.06.008.. doi:10.1016/j.neures.2023.06.008.

RELEVANT LINKS

Blast Injury Protocol

https://dx.doi.org/10.17504/protocols.io.dm6gpjk9jgzp/v1

NOTES

DATASET INFO

Contact: VandeVord Pamela (pvord@vt.edu)

Lab: PRECISE-TBI Lab: VandeVord TNT Lab_Salem VA

ODC-TBI Accession:1014

Records in Dataset: 14

Fields per Record: 111

Last updated: 2024-04-15

Date published: 2024-04-15

Downloads: 2

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

Virginia Tech Institute for Critical Technology and Applied Science , Virginia Space Grant Consortium

CONTRIBUTORS

Norris, Carly

Virginia Tech

Murphy, Susan F.

Virginia Tech

VandeVord, Pamela J.

Virginia Tech

Weatherbee, Justin

Virginia Tech